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1Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research,Università degli Studi di Milano, 2Department of Veterinary Medicine,University of Sassari, 3Department of Agricultural and Environmental Sciences – Production, Landscape, Agroenergy and Center for Stem Cell Research,Università degli Studi di Milano
Chapters
- 00:04Introduction
- 01:11Fibroblast Exposure to 5-aza-CR
- 02:28Fibroblast Encapsulation in PTFE Micro-Bioreactors
- 04:05Culture in PTFE Micro-Bioreactors of Epigenetically Erased Cells
- 05:00Results: Epigenetically Erased Cells Encapsulated in PTFE Micro-Bioreactors Show High Level and Long-term Maintenance of Pluripotency-Related Gene Expression
- 07:33Conclusion
Aquí presentamos un método que combina el uso del borrado epigenético químico con señales relacionadas con la mecanodetección para generar eficientemente células pluripotentes de mamíferos, sin necesidad de transfección génica o vectores retrovirales. Esta estrategia es, por lo tanto, prometedora para la medicina traslacional y representa un avance notable en la tecnología de organoides de células madre.
