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Etablering och genetisk manipulering av Murine Hepatocyte Organoids
JoVE Journal
Biology
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JoVE Journal Biology
Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
DOI:

14:54 min

February 12, 2022

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Chapters

  • 00:01Introduction
  • 00:47Murine Hepatocyte Isolation by Liver Perfusion and Digestion
  • 02:47Hepatocyte Organoids Culture
  • 04:29Single Cells from Hepatocyte Organoids
  • 07:22siRNA or Plasmid Transfection
  • 12:58Representative Results
  • 13:58Conclusion

Summary

Automatic Translation

Ett långsiktigt ex vitro 3D organoid kultursystem inrättades från mus hepatocyter. Dessa organoider kan passeras och genetiskt manipuleras av lentivirus infektion av shRNA/ektopisk konstruktion, siRNA transfection och CRISPR-Cas9 teknik.

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