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JoVE Journal
Biochemistry

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纳米微分扫描荧光仪,用于在基于碎片的铅发现中筛选
 
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纳米微分扫描荧光仪,用于在基于碎片的铅发现中筛选

Article DOI: 10.3791/62469-v
May 16th, 2021

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Summary May 16th, 2021

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Click here for the English version.

监测目标蛋白(热移位检测、TSA)融化温度的变化是筛选几百种化合物片段库的有效方法。我们提出了一个TSA协议,实施机器人辅助纳米微分扫描荧光仪(nano-DSF),用于监测内在色氨酸荧光和光反散射,用于碎片筛选。

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