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Kombineret mekanisk og enzymatisk dissociation af musehjernens hippocampale væv
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Neuroscience
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JoVE Journal Neuroscience
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Kombineret mekanisk og enzymatisk dissociation af musehjernens hippocampale væv

DOI:
10.3791/63007-v

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07:14 min

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October 21, 2021

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, , , , ,
1Division of Radiation Health,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Chapters

  • 00:04Introduction
  • 00:50Perfusion
  • 02:01Dissection
  • 03:11Adult Brain Dissociation
  • 04:15Debris Removal
  • 05:28Live and Dead Staining
  • 06:00Results: Flow Cytometry Analysis Following Automated Mechanical Dissociation and Enzymatic Digestion of Mouse Brain Hippocampal Tissue
  • 06:51Conclusion

Summary

Automatic Translation

Automatic Translation

Denne neurale celle dissociationsprotokol er beregnet til prøver med en lav mængde udgangsmateriale og giver en meget levedygtig enkeltcellesuspension til downstream-analyse med valgfri fikserings- og farvningstrin.

Tags

Neural DissociationSingle-cell SuspensionMouse BrainHippocampusEnzymatic DissociationMechanical DissociationPerfusionBrain ExtractionSample Preparation

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