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Journal / Biochemistry / Immunopeptidomikk: Isolering av mus og human MHC…
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Immunopeptidomikk
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Immunopeptidomikk: Isolering av mus og human MHC klasse I- og II-assosierte peptider for massespektrometrianalyse

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Her presenterer vi en protokoll for rensing av MHC klasse I og klasse II peptidkomplekser fra mus- og menneskecellelinjer som gir immunopeptidomiske data av høy kvalitet. Protokollen fokuserer på prøvepreparering ved hjelp av kommersielt tilgjengelige antistoffer.

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