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Bioengineering
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一种研究炎症期间白细胞-内皮细胞相互作用的微生理系统
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
Please note that all translations are automatically generated.
Click here for the English version.
一种研究炎症期间白细胞-内皮细胞相互作用的微生理系统
DOI:
10.3791/63312-v
•
12:55 min
•
December 09, 2021
•
Qingliang Yang
,
Jordan C. Langston
,
Yuan Tang
,
Balabhaskar Prabhakarpandian
,
Laurie E. Kilpatrick
,
Mohammad F. Kiani
2,6
1
Department of Mechanical Engineering
,
Temple University
,
2
Department of Bioengineering
,
Temple University
,
3
Department of Bioengineering
,
University of Toledo
,
4
Biomedical Technology
,
CFD Research Corporation
,
5
Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine
,
Temple University
,
6
Department of Radiation Oncology, Lewis Katz School of Medicine
,
Temple University
Chapters
00:04
Introduction
01:13
Priming and Coating the Device with Human Fibronectin
03:01
Seeding Biomimetic Microfluid Assay (bMFA) with HLMVEC
04:37
Cell Culture Under Flow for 48 Hours
05:26
Cytokine and/or Therapeutic Treatment
06:19
Neutrophil Adhesion and Migration Experiment with bMFA
08:33
Acquire Image
09:59
Results: Biomimetic Microfluid Assay to Study Leukocyte-Endothelial Cell Interactions
12:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
在该协议中,仿生微流体测定法可用于研究炎症性疾病中的白细胞 - 内皮细胞相互作用,该测定可以再现生理相关的微血管环境并重现整个白细胞粘附/迁移级联反应。
Tags
Microphysiological System
Leukocyte-endothelial Cell Interaction
Inflammation
Sepsis
Vascular Endothelium Barrier
Leukocyte Trafficking
Organ Damage
Biomimetic Microfluidic Assay
Primary Human Cells
Clinically Relevant Patient Samples
Therapeutic Screening
Neutrophil Rolling
Adhesion
Spreading
Migration
Human Fibronectin
Degassing
Human Lung Microvascular Endothelial Cells
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