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Een microfysiologisch systeem om leukocyten-endotheelcelinteractie tijdens ontsteking te bestuderen
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1Department of Mechanical Engineering,Temple University, 2Department of Bioengineering,Temple University, 3Department of Bioengineering,University of Toledo, 4Biomedical Technology,CFD Research Corporation, 5Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine,Temple University, 6Department of Radiation Oncology, Lewis Katz School of Medicine,Temple University
Chapters
- 00:04Introduction
- 01:13Priming and Coating the Device with Human Fibronectin
- 03:01Seeding Biomimetic Microfluid Assay (bMFA) with HLMVEC
- 04:37Cell Culture Under Flow for 48 Hours
- 05:26Cytokine and/or Therapeutic Treatment
- 06:19Neutrophil Adhesion and Migration Experiment with bMFA
- 08:33Acquire Image
- 09:59Results: Biomimetic Microfluid Assay to Study Leukocyte-Endothelial Cell Interactions
- 12:06Conclusion
In dit protocol wordt een biomimetische microfluïde assay, die een fysiologisch relevante microvasculaire omgeving kan reproduceren en de gehele leukocytenadhesie / migratiecascade kan reproduceren, gebruikt om leukocyten-endotheelcelinteracties bij ontstekingsziekten te bestuderen.
Tags
Microphysiological SystemLeukocyte-endothelial Cell InteractionInflammationSepsisVascular Endothelium BarrierLeukocyte TraffickingOrgan DamageBiomimetic Microfluidic AssayPrimary Human CellsClinically Relevant Patient SamplesTherapeutic ScreeningNeutrophil RollingAdhesionSpreadingMigrationHuman FibronectinDegassingHuman Lung Microvascular Endothelial Cells
