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用于表征成人型弥漫性浸润性胶质瘤微环境的数字空间剖析
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Cancer Research
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JoVE Journal Cancer Research
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
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用于表征成人型弥漫性浸润性胶质瘤微环境的数字空间剖析

DOI:
10.3791/63620-v

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09:17 min

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September 13, 2022

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, , , , ,
1Department of Neurology,Dartmouth-Hitchcock Medical Center, 2Department of Pathology and Laboratory Medicine,Dartmouth-Hitchcock Medical Center, 3Department of Neurosurgery,Dartmouth-Hitchcock Medical Center, 4Dartmouth Cancer Center,Dartmouth-Hitchcock Medical Center

Chapters

  • 00:04Introduction
  • 01:08Preparation of Slides and Semi‐Automated IHC System
  • 02:50Antibody Incubation and Nuclei Staining
  • 03:39Fluorescence Visualization, ROI Identification, and UV Photocleavage
  • 05:17Protein Readout and Data Analysis
  • 07:51Results: Digital Spatial Profiling on Glioblastoma Samples
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

蛋白质组学失调在弥漫性浸润性胶质瘤的扩散中起重要作用，但几种相关蛋白质仍未鉴定。数字空间处理（DSP）提供了一种高效、高通量的方法，用于表征可能导致浸润性胶质瘤侵袭和迁移的候选蛋白的差异表达。

Tags

Digital Spatial ProfilingDSPProtein QuantificationMicroenvironmentAdult-type Diffusely Infiltrating GliomaMultiplexingRegions Of InterestOncologyTarget VariabilityMalignant GrowthInvasionImmunohistochemistryBlocking SolutionScanningHydrophobic Barrier

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