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Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

DOI:
10.3791/64175-v

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07:25 min

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July 07, 2022

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, ,
1Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Chapters

  • 00:04Introduction
  • 00:55Spinal Cord Tissue Dissection
  • 01:34Magnetic Activated Cell Sorting (MACS)
  • 02:39Seeding of Astrocytes for Reprogramming
  • 04:25Reprogramming of Astrocytes (7‐Day Analysis)
  • 05:29Results: Murine Astrocyte Cultures to Study Direct Neuronal Reprogramming
  • 06:37Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a detailed protocol to generate highly enriched cultures of astrocytes derived from different regions of the central nervous system of postnatal mice and their direct conversion into functional neurons by the forced expression of transcription factors.

Tags

Keywords: AstrocytesNeuronal ReprogrammingCell IsolationSpinal CordCell CultureAstrocyte CulturePoly-D-LysineEGFBFGF

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