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Journal / Immunology and Infection / Effektiv transfektion av in vitro-transkriberat…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Effektiv transfektion av in vitro-transkriberat mRNA i odlade celler med användning av peptid-poloxaminnanopartiklar
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Effektiv transfektion av in vitro-transkriberat mRNA i odlade celler med användning av peptid-poloxaminnanopartiklar

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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En självmonterad peptid-poloxaminnanopartikel (PP-sNp) utvecklas med hjälp av en mikrofluidisk blandningsanordning för att kapsla in och leverera in vitro transkriberat budbärar-RNA. Det beskrivna mRNA / PP-sNp kan effektivt transfektera odlade celler in vitro.

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Immunologi och infektion utgåva 186 Genleverans in vitro transkriberat budbärar-RNA leveranssystem poloxamin nanopartikel
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