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Chapters
- 00:05Title
- 01:47PEGDM Synthesis
- 03:12Culture, Infection, and Aggregation of Cells
- 04:42Encapsulation of Cells in PEGDM
- 06:39Hypoxia Tracking
- 07:12Representative Hydrogels and Fluorescent Hypoxic Signaling
- 08:39Conclusion
A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.
Tags
TrackingHypoxic SignalingEncapsulated Cell AggregatesDiabetes Mellitus Type 1Pancreatic Beta-cellsInsulin ProductionHyperglycemiaExogenous Insulin InjectionTransplantationNormoglycemiaImmune SystemEncapsulationBiologically-derived MaterialsAlginateAgaroseInflammationFibrotic OvergrowthNutrient TransportOxygen TransportSynthetic Poly(ethylene Glycol)PEG-based HydrogelsPhoto-crosslinking ReactionUV DegradationPhotoinitiatorIrgacure 2959Free Radicals
