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The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
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Chapters
- 00:05Title
- 01:35Preparation of Citrobacter rodentium Inoculum and Oral Gavage of Mice
- 03:03Measuring Colonic Epithelial Barrier Permeability in C. rodentium-infected Mice
- 04:18Measurement of Bacterial Load in Tissues of C. rodentium-infected Mice
- 06:06Histological Assessment and Immunofluorescence Staining of Infected Colon Tissues
- 08:02Results: Representative Overnight Culture Infection Data from a Frozen Glycerol Stock
- 10:14Conclusion
Citrobacter rodentium infection provides a valuable model to study enteric bacterial infections as well as host immune responses and colitis in mice. This protocol outlines the measurement of barrier integrity, pathogen load and histological damage allowing for the thorough characterization of pathogen and host contributions to murine infectious colitis.
