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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
JoVE Journal
Biology
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JoVE Journal Biology
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
DOI:

11:46 min

May 26, 2014

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Chapters

  • 00:05Title
  • 01:36Preparation of DNA Mixes
  • 04:25Transfection
  • 05:59Measurement of BRET Signal
  • 07:14Results: Assessing FOXP2 Protein-protein Interactions Using BRET
  • 11:09Conclusion

Summary

Automatic Translation

Interactions between proteins are fundamental to all cellular processes. Using Bioluminescence Resonance Energy Transfer, the interaction between a pair of proteins can be monitored in live cells and in real time. Furthermore, the effects of potentially pathogenic mutations can be assessed.

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