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JoVE Journal Biology
Generation af
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
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Generation af

Article DOI: 10.3791/51934-v • 13:31 min • October 31st, 2014
October 31st, 2014 •
1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University

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Enterobacter sp. YSU vokser i glucose minimale salte medium. Auxotrofer genereres ved at omdanne den med en transposome som tilfældigt indsætter sig selv i værtens genom. Mutanter findes ved replikaudpladning fra komplekst medium til minimalmedium. Afbrudte gener identificeres ved gen-redning og sekventering.

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Mikrobiologi auxotrofen transposome transposon mutagenese replikaudpladning glucose minimal medium kompleks medium,
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