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High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
JoVE Journal
Biology
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JoVE Journal Biology
High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
DOI:

13:13 min

September 23, 2014

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Chapters

  • 00:05Title
  • 01:43Passaging and Maintenance of Undifferentiated hPSCs in Monolayer Culture
  • 05:18Cardiomyocyte Induction of hPSCs by Selective Modulation of the Wnt Pathway
  • 08:22Fixation and Permeabilization of Cells for Intracellular Antigen Staining
  • 09:25Preparation of Cells for Flow Cytometry and Flow Cytometric Analysis
  • 10:34Characterization of Cardiomyocytes by Flow Cytometry and Electrophysiology
  • 12:26Conclusion

Summary

Automatic Translation

The article describes the detailed methodology to efficiently differentiate human pluripotent stem cells into cardiomyocytes by selectively modulating the Wnt pathway, followed by flow cytometry analysis of reference markers to assess homogeneity and identity of the population.

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