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1Department of Biochemistry,Medical College of Wisconsin, 2Stanford Cardiovascular Institute,Stanford University School of Medicine, 3Department of Anesthesiology,Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine,Hong Kong University, 5Division of Cardiology,Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center,Medical College of Wisconsin
Chapters
- 00:05Title
- 01:43Passaging and Maintenance of Undifferentiated hPSCs in Monolayer Culture
- 05:18Cardiomyocyte Induction of hPSCs by Selective Modulation of the Wnt Pathway
- 08:22Fixation and Permeabilization of Cells for Intracellular Antigen Staining
- 09:25Preparation of Cells for Flow Cytometry and Flow Cytometric Analysis
- 10:34Characterization of Cardiomyocytes by Flow Cytometry and Electrophysiology
- 12:26Conclusion
The article describes the detailed methodology to efficiently differentiate human pluripotent stem cells into cardiomyocytes by selectively modulating the Wnt pathway, followed by flow cytometry analysis of reference markers to assess homogeneity and identity of the population.
