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En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
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En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

DOI:
10.3791/53718-v

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08:58 min

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February 25, 2016

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, , , ,
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg

Chapters

  • 00:05Title
  • 00:52Preparing the Organ Bath System
  • 02:01Isolation of Mouse Aortas
  • 03:03DHE and DAF-2DA Staining
  • 04:47En Face Mounting and Confocal Microscopic Imaging
  • 06:42Results: eNOS-uncoupling in Obese Mice
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

In this article we describe an adapted relatively easy method using the fluorescence dye diaminofluorescein-2 diacetate (DAF-2DA) and dihydroethidium (DHE) for en face simultaneous detection and visualization of intracellular nitric oxide (NO) and superoxide anion (O2.−) respectively, in freshly isolated intact aortas of an obesity mouse model.

Tags

Nitric OxideSuperoxideEndothelial LayerIntact ArteriesEn Face DetectionDAF-2DADHECardiovascular PhysiologyEndothelial DysfunctionENOS UncouplingOrgan Bath SystemKrebs-Ringer Bicarbonate BufferAortic RingsDissectionFluorescent Staining

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