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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
DOI:

10:24 min

March 03, 2017

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Chapters

  • 00:05Title
  • 00:41Cell Culture and HIV-1 Infection
  • 02:11Exosome Isolation
  • 03:42Protein Extraction
  • 04:58Proteomic Data Analysis
  • 05:43Bioinformatics Verification and Characterization
  • 08:20Results: SILAC Is an Effective Method for Analyzing the Effects of HIV-1 Infection on Host Exosomal Proteomes
  • 09:08Conclusion

Summary

Automatic Translation

Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

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