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Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
DOI:

10:36 min

November 06, 2017

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Chapters

  • 00:05Title
  • 01:11Brain Dissection and Isolation of the Cerebellum
  • 03:11Mouse Cerebellar Granule Neuron Isolation and Culturing
  • 06:38Modeling Neuronal Injury
  • 08:09Results: Representative Images of Cerebellar Granule Neurons Infected with Different MOIs
  • 09:36Conclusion

Summary

Automatic Translation

This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.

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