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Chapters
- 00:05Title
- 00:47PDMS Device Casting
- 01:54Device Punching and Bonding
- 03:19Microfluidic Plumbing Preparation
- 05:35Device Loading
- 06:47Device Assembly, Equilibration, and Mounting
- 08:47Results: Single-cell Microfluidic Analysis of Bacillus subtilis
- 10:05Conclusion
We present a method for the microfluidic analysis of individual bacterial cell lineages using Bacillus subtilis as an example. The method overcomes shortcomings of traditional analytical methods in microbiology by allowing observation of hundreds of cell generations under tightly controllable and uniform growth conditions.
