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Immunology and Infection
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Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
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Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

DOI:
10.3791/60723-v

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08:15 min

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January 31, 2020

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, , , , , , , , ,
1Department of Clinical Medicine,Aarhus University, 2Department of Infectious Diseases,Aarhus University Hospital, 3Department of Biomedicine,Aarhus University, 4Department of Gynecology and Obstetrics,Aarhus University Hospital, 5Department of Biology,University of Nebraska at Omaha

Chapters

  • 00:04Title
  • 00:50Cord Blood CCR5Δ32 Genetic Variant Screening
  • 01:50Intravaginal HIV Exposure
  • 03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
  • 04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
  • 05:42Results: Representative HIV infection and Treatment DATA
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA quantification.

Tags

Humanized NOG MiceIntravaginal HIV ExposureHIV InfectionPreclinical HIV ResearchViral StrainsHIV InterventionsCCR5 VariantViral RNA QuantificationCord Blood ScreeningCCR5 Delta 32HIV Exposure Protocol

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