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Journal / Biology / 5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3…
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JoVE Journal Biology
5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol voor celcyclusprogressieanalyse in drosophila neurale stamcellen
An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos
Journal (Biology)
An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos
Detection of Protein Ubiquitination
Journal (Biology)
Detection of Protein Ubiquitination
 
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5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol voor celcyclusprogressieanalyse in drosophila neurale stamcellen

Article DOI: 10.3791/62642-v • 09:34 min • May 4th, 2021
May 4th, 2021 •
1,2, 1
1Cell Biology, University of Bern, 2Department of life sciences and medicine, University of Luxembourg

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Celcyclusanalyse met 5-ethynyl-2'-deoxyuridine (EdU) en fosfo-histon H3 (pH3) etikettering is een procedure in meerdere stappen die uitgebreide optimalisatie kan vereisen. Hier presenteren we een gedetailleerd protocol dat alle stappen voor deze procedure beschrijft, inclusief beeldanalyse en kwantificering om cellen in verschillende celcyclusfasen te onderscheiden.

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Biologie Nummer 171
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