Orthotopic Injection into the Mammary Fat Pad: Establishing Breast Cancer in Mice

Abstract

Source: Zhang, G. L., et al. Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad. J. Vis. Exp. (2019).

In this article we discuss how to establish a breast cancer model in female mice by injecting breast cancer cells into the mammary fat pad. The example protocol demonstrates the orthotopic injection.

Protocol

Animal experiments were conducted in accordance with the Provision and General Recommendation of Chinese Experimental Animal Administration Legislation and were approved by the Institution of Animal Care and Use Committee of Capital Medical University (Ref no. AEEI-2014-052). Female Balb/c mice aged 6 to 8 weeks were used.

1. Preparation of the Cells and Animals

1. One day before the operation, shave the hair around the fourth nipples to expose the operating area.
   1. Use one hand to hold the mouse tightly at the back to make sure the mouse will not move freely, turn up the mouse to expose its belly to the operator, and then use another hand to s....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
anesthesia machine	Midmark Corporation, Dayton, OH, USA	Matrx VMS	anesthesia
 isoflurane	Hebei yipin chemical reagents  company	O21400	anesthesia
1 mL syringe	Becton, Dickinson and  Company	A257	cell injection
hair removal cream	Veet	-	hair removal cream
Carbomer Eye Gel	Dr.GerhardMannChem-Pharm.FabrikGmbH	-	ophthalmic ointment