Touch Habituation Assay: A Method to Study Non-Associative Learning in C. elegans
Published: April 30, 2023
Abstract
Source: Mersha, M. D., et al. Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants. J. Vis. Exp. (2018).
This video introduces the touch habituation assay, a C. elegans behavioral method of assessing mechanosensation and quantifying time to habituation.
Protocol
The following protocol is an excerpt from Mersha et al. Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants, J. Vis. Exp. (2018) Anterior Touch Habituation Assay Transfer approximately 10 synchronized young adult worms to new unseeded NGM plates using a fire-sterilized 30 G platinum wire pick. Leave worms undisturbed for 5 min to allow them time to acclimatize to the new plate. For the habituation response, …
Materials
| EtOH | Sigma-Aldrich | 64-17-5 | |
| N2 (Wild-type C. elegans) worms | CGC | Repository at U of Minnesota | |
| Platinum wire pick | Genesee Scientific | 59-AWP | |
| Petri plates | Fisher | 07-202-011 | |
| Dissection Microscope | AmScope | SM-2TYY |
Cite This Article
Touch Habituation Assay: A Method to Study Non-Associative Learning in C. elegans. J. Vis. Exp. (Pending Publication), e20160, doi: (2023).