Calcium Imaging: En metode til at visualisere neurale aktivitet i Live C. elegans
Published: April 30, 2023
Abstract
Kilde: Turek, M. et al. Agarose Mikrochambers for Langsigtet Calcium Imaging af Caenorhabditis elegans. J. Vis. Exp. (2015).
Denne video introducerer begreberne bag calcium imaging og indeholder et eksempel protokol for time-lapse billeddannelse af orme indeholdt i støbt agarose brønde.
Protocol
Denne protokol er et uddrag fra Turek et al, Agarose Microchambers for Langsigtet Calcium Imaging af Caenorhabditis elegans, J. Vis. Exp. (2015). 1. Calcium imaging Brug transgene stammer, der udtrykker genetisk kodede calciumsensorer som HBR16 (goeIs5[pnmr-1::SL1-GCaMP3.35-SL2::unc-54-3’UTR, unc-119(+)]). Br…
Materials
| LED system | CoolLED | pE-2 | |
| Compound microscope | Nikon | TiE | |
| EMCCD camera | Andor | iXon 897 | now sold as iXon Ultra 897 |
| Z-stage for the microscope | Prior | Nano Scan Z | |
| X-Y stage for the microscope | Prior | Proscan III | |
| Red light filter | Chroma | ET660/50m | |
| 35 mm Petri dish | Falcon | Falcon Disposable Petri Dishes, Sterile, Corning |
Cite This Article
Calcium Imaging: A Method to Visualize Neural Activity in Live C. elegans. J. Vis. Exp. (Pending Publication), e20164, doi: (2023).