Photomotor Response Assay: A Method to Measure the Behavioral Response of Larval Zebrafish to a Sudden Change in Lighting Condition
Published: April 30, 2023
Abstract
Source: Steele, W. B., et al. Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine. J. Vis. Exp. (2018).
This video describes the photomotor response assay of zebrafish and fathead minnow larvae using an automated tracking software. The protocol measures the behavioral response of the larval fish following a sudden change in lighting condition.
Protocol
1. Calibration of Video-tracking Parameters Prior to behavioral measures, set observation and calibration parameters in the video track software (see Table of Materials). Place a well plate in the recording chamber with at least 1 larval fish in an individual well. Use the plate and associated fish as representations to set calibration parameters. In the video track software, click "File | Generate Protocol", which will open a “protocol creation wizardR…
Materials
| 48-well plates | Fisher Scientific | 08-772-52 | Larval zebrafish behavioral recording chambers |
| 24-well plates | VWR | 10062-896 | Larval fathead minnow behavioral recording chambers |
Cite This Article
Photomotor Response Assay: A Method to Measure the Behavioral Response of Larval Zebrafish to a Sudden Change in Lighting Condition. J. Vis. Exp. (Pending Publication), e20171, doi: (2023).