This video describes the photomotor response assay of zebrafish and fathead minnow larvae using an automated tracking software. The protocol measures the behavioral response of the larval fish following a sudden change in lighting condition.
Protocol
1. Calibration of Video-tracking Parameters Prior to behavioral measures, set observation and calibration parameters in the video track software (see Table of Materials). Place a well plate in the recording chamber with at least 1 larval fish in an individual well. Use the plate and associated fish as representations to set calibration parameters. In the video track software, click "File | Generate Protocol", which will open a “protocol creation wizardR…
Photomotor Response Assay: A Method to Measure the Behavioral Response of Larval Zebrafish to a Sudden Change in Lighting Condition. J. Vis. Exp. (Pending Publication), e20171, doi: (2023).