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双光子现场斑马鱼的胚胎干切断和时间推移共焦成像
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Biology
Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
Please note that all translations are automatically generated.
Click here for the English version.
双光子现场斑马鱼的胚胎干切断和时间推移共焦成像
DOI:
10.3791/1129-v
•
12:21 min
•
February 16, 2009
•
Georgeann S. O’Brien
,
Sandra Rieger
,
Seanna M. Martin
,
Ann M. Cavanaugh
,
Carlos Portera-Cailliau
,
Alvaro Sagasti
1
Department of Molecular Cell and Developmental Biology
,
University of California, Los Angeles
,
2
Departments of Neurology and Neurobiology
,
University of California, Los Angeles
Chapters
00:00
Title
00:47
Introduction
01:28
Mounting Zebrafish Embryos for Long-Term Imaging
03:25
Two-photon axotomy using a custom built two-photon microscope with a Chameleon Ti-Sapphire laser
04:42
Two-photon axotomy on Zeiss 510 confocal/ two-photon microscope
06:52
Confocal time-lapse imaging on Zeiss LSM 510
10:56
Outcome and Results
11:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个用于安装长期成像,双光子成像和组织损伤的技术,和时间推移共焦成像的斑马鱼胚胎的方法。
Tags
Two-photon Axotomy
Time-lapse Confocal Imaging
Zebrafish Embryos
Cellular Mechanisms Of Development
Long-term Imaging
Automated Image Capture
Confocal Microscope
Peripheral Sensory Axons
Femtosecond Laser
Two-photon Microscope
GFP Expression
Sensory Neuron Regeneration
Anesthetized Embryos
Agarose Positioning
Imaging Chamber
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