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双光子现场斑马鱼的胚胎干切断和时间推移共焦成像

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Biology

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JoVE Journal Biology

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Please note that all translations are automatically generated. Click here for the English version.

双光子现场斑马鱼的胚胎干切断和时间推移共焦成像

DOI:

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12:21 min

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February 16, 2009

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Georgeann S. O’Brien, Sandra Rieger, Seanna M. Martin, Ann M. Cavanaugh, Carlos Portera-Cailliau, Alvaro Sagasti

¹Department of Molecular Cell and Developmental Biology,University of California, Los Angeles, ²Departments of Neurology and Neurobiology,University of California, Los Angeles

Chapters

00:00Title
00:47Introduction
01:28Mounting Zebrafish Embryos for Long-Term Imaging
03:25Two-photon axotomy using a custom built two-photon microscope with a Chameleon Ti-Sapphire laser
04:42Two-photon axotomy on Zeiss 510 confocal/ two-photon microscope
06:52Confocal time-lapse imaging on Zeiss LSM 510
10:56Outcome and Results
11:44Conclusion

Summary

Automatic Translation

在这里，我们描述了一个用于安装长期成像，双光子成像和组织损伤的技术，和时间推移共焦成像的斑马鱼胚胎的方法。

Tags

Two-photon Axotomy Time-lapse Confocal Imaging Zebrafish Embryos Cellular Mechanisms Of Development Long-term Imaging Automated Image Capture Confocal Microscope Peripheral Sensory Axons Femtosecond Laser Two-photon Microscope GFP Expression Sensory Neuron Regeneration Anesthetized Embryos Agarose Positioning Imaging Chamber

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