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双光子现场斑马鱼的胚胎干切断和时间推移共焦成像
JoVE Journal
Biology
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JoVE Journal Biology
Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
DOI:

12:21 min

February 16, 2009

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Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:28Mounting Zebrafish Embryos for Long-Term Imaging
  • 03:25Two-photon axotomy using a custom built two-photon microscope with a Chameleon Ti-Sapphire laser
  • 04:42Two-photon axotomy on Zeiss 510 confocal/ two-photon microscope
  • 06:52Confocal time-lapse imaging on Zeiss LSM 510
  • 10:56Outcome and Results
  • 11:44Conclusion

Summary

Automatic Translation

在这里,我们描述了一个用于安装长期成像,双光子成像和组织损伤的技术,和时间推移共焦成像的斑马鱼胚胎的方法。

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