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Journal
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Biology
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可视化抗原特异性CD4 + T细胞的MHC II类四聚四
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JoVE Journal
Biology
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Please note that all translations are automatically generated.
Click here for the English version.
可视化抗原特异性CD4 + T细胞的MHC II类四聚四
DOI:
10.3791/1167-v
•
15:42 min
•
March 06, 2009
•
Eddie A. James
,
Rebecca LaFond
,
Ivana Durinovic-Bello
,
William Kwok
1
Tetramer Core Laboratory
,
Benaroya Research Institute
,
2
Kwok Laboratory
,
Benaroya Research Institute
,
3
Nepom Laboratory
,
Benaroya Research Institute
Chapters
00:00
Title
00:09
Introduction
01:05
T Cell Staining
03:21
Reagents and Equipment
05:44
Assemble Materials and Equipment at Workspace
06:04
Step 1: PBMC Isolation
08:51
Step 2: CD4+ Cell Separatioin
11:01
Step 3: In Vitro Expansion Culture
13:34
Step 4: Visualizing T Cells by Tetramer Staining
15:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此过程演示的纯化和体外扩增抗原特异性CD4 +从整体外周血T细胞及其可视化使用MHC II类四聚体。四聚体,允许直接与单个抗原特异性T细胞和定义MHC II类限制性的可视化。
Tags
CD4+ T Cells
MHC Class II Tetramers
Antigen Specific
Flow Cytometry
Peptide Loaded MHC
T-cell Receptor
Streptavidin
Staining Reagents
In Vitro Amplification
Mononuclear Cells
Peripheral Blood
Ficoll Underlay
Negative Selection
Biotinylated Antibody Cocktail
Magnetic Beads
Adherent Cells
Antigen Presenting Cells
IL-2
Surface Antibodies
Flow Cytometry Analysis
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