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Chapters
- 00:00Title
- 00:09Introduction
- 01:05T Cell Staining
- 03:21Reagents and Equipment
- 05:44Assemble Materials and Equipment at Workspace
- 06:04Step 1: PBMC Isolation
- 08:51Step 2: CD4+ Cell Separatioin
- 11:01Step 3: In Vitro Expansion Culture
- 13:34Step 4: Visualizing T Cells by Tetramer Staining
- 15:33Conclusion
Bu prosedür, arıtma gösteriyor ve in vitro antijen spesifik CD4 + T hücreleri, tüm periferik kan ve MHC sınıf II tetramers kullanarak görselleştirme genişleme. Tetramers tek bir antijen özgüllüğü ve tanımlanan MHC sınıf II kısıtlama ile T hücrelerinin doğrudan görselleştirme izin verir.
Tags
CD4+ T CellsMHC Class II TetramersAntigen SpecificFlow CytometryPeptide Loaded MHCT-cell ReceptorStreptavidinStaining ReagentsIn Vitro AmplificationMononuclear CellsPeripheral BloodFicoll UnderlayNegative SelectionBiotinylated Antibody CocktailMagnetic BeadsAdherent CellsAntigen Presenting CellsIL-2Surface AntibodiesFlow Cytometry Analysis
