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Direkt observation av enzymer Replikera DNA med en enda molekyl DNA Stretching analys
JoVE Journal
Biology
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JoVE Journal Biology
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
DOI:

17:03 min

March 23, 2010

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Chapters

  • 00:00Title
  • 00:51Introduction
  • 01:18Preparation of the DNA Replication Template
  • 03:41Bead Preparation
  • 05:28Preparing Functionalized Coverslips
  • 08:31Flow Chamber Assembly
  • 11:34Single-Molecule Replication Experiment
  • 15:41Single-Molecular Replication Data Analysis and Representative Results
  • 16:34Conclusion

Summary

Automatic Translation

Vi beskriver en metod för att observation realtid replikering av enskilda DNA-molekyler förmedlas av proteiner från bakteriofag replikering systemet.

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