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移植绿色荧光蛋白表达对视网膜和大脑发育的实时成像嵌合斑马鱼胚胎的卵裂球
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Biology
Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
移植绿色荧光蛋白表达对视网膜和大脑发育的实时成像嵌合斑马鱼胚胎的卵裂球
DOI:
10.3791/1924-v
•
08:09 min
•
July 19, 2010
•
Jian Zou
,
Xiangyun Wei
2
1
Department of Ophthalmology
,
University of Pittsburgh
,
2
Department of Microbiology and Molecular Genetics
,
University of Pittsburgh
Chapters
00:00
Title
00:32
Introduction
00:59
Day 1: Setup
03:28
Day 2: Blastomere Transplantation
05:55
Day 3: Embedding and Live Imaging of Mosaic Embryos
06:48
Confocal Imaging
07:17
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们展示了协议,实时成像胚胎发育过程中的细胞行为产生的嵌合斑马鱼的胚胎。
Tags
Transplantation
GFP-expressing Blastomeres
Live Imaging
Retinal Development
Brain Development
Chimeric Zebrafish Embryos
Cell-cell Interactions
Genetic Background
Embryonic Transparency
Morphogenesis
Cellular Level
Transplantation Needles
GFP-negative Hosts
Confocal Microscopy
Embryonic Development
GFP Labeling
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