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移植绿色荧光蛋白表达对视网膜和大脑发育的实时成像嵌合斑马鱼胚胎的卵裂球

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Biology

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JoVE Journal Biology

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

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移植绿色荧光蛋白表达对视网膜和大脑发育的实时成像嵌合斑马鱼胚胎的卵裂球

DOI:

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08:09 min

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July 19, 2010

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Jian Zou, Xiangyun Wei²

¹Department of Ophthalmology,University of Pittsburgh, ²Department of Microbiology and Molecular Genetics,University of Pittsburgh

Chapters

00:00Title
00:32Introduction
00:59Day 1: Setup
03:28Day 2: Blastomere Transplantation
05:55Day 3: Embedding and Live Imaging of Mosaic Embryos
06:48Confocal Imaging
07:17Representative Results

Summary

Automatic Translation

我们展示了协议，实时成像胚胎发育过程中的细胞行为产生的嵌合斑马鱼的胚胎。

Tags

Transplantation GFP-expressing Blastomeres Live Imaging Retinal Development Brain Development Chimeric Zebrafish Embryos Cell-cell Interactions Genetic Background Embryonic Transparency Morphogenesis Cellular Level Transplantation Needles GFP-negative Hosts Confocal Microscopy Embryonic Development GFP Labeling

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