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Die Transplantation von GFP-exprimierenden Blastomeren für Live Imaging von Retinal und Entwicklung des Gehirns in Chimäre Zebrafischembryonen
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Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
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Die Transplantation von GFP-exprimierenden Blastomeren für Live Imaging von Retinal und Entwicklung des Gehirns in Chimäre Zebrafischembryonen

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10,581 Views

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08:09 min

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July 19, 2010

DOI:

10.3791/1924-v

DOI:
10.3791/1924-v

•

08:09 min
July 19, 2010

•

1 Views
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1Department of Ophthalmology,University of Pittsburgh, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh

Summary

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Wir zeigen, ein Protokoll zu chimären Zebrafischembryonen für Live-Imaging-zelluläres Verhalten während der Embryogenese zu generieren.

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TransplantationGFP-expressing BlastomeresLive ImagingRetinal DevelopmentBrain DevelopmentChimeric Zebrafish EmbryosCell-cell InteractionsGenetic BackgroundEmbryonic TransparencyMorphogenesisCellular LevelTransplantation NeedlesGFP-negative HostsConfocal MicroscopyEmbryonic DevelopmentGFP Labeling

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Cite this Article

Zou, J., Wei, X. Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos. J. Vis. Exp. (41), e1924, doi:10.3791/1924 (2010).

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