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Immunology and Infection
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HIV - 1使用转速依赖的慢病毒表达载体的绿色荧光蛋白阳性细胞的特定标记
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
Please note that all translations are automatically generated.
Click here for the English version.
HIV - 1使用转速依赖的慢病毒表达载体的绿色荧光蛋白阳性细胞的特定标记
DOI:
10.3791/2198-v
•
09:53 min
•
September 23, 2010
•
Jia Guo*
1
,
Clinton Enos*
1
,
Yuntao Wu
1
National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology
,
George Mason University
Chapters
00:00
Title
01:15
Plasmid Transfection to Produce Rev-dependent Lentiviral Particles
04:24
Concentrating Viral Particles and Determining Viral Titer
06:15
Marking HIV-1 Positive Cells
08:25
Identifying HIV-positive Cells
09:30
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
我们已经开发出一种慢病毒载体,拥有,除了达反应的LTR,冯应答元件(RRE)报告基因的表达在HIV - 1达和冯依赖的方式,可以调节。向量允许通过活细胞GFP表达的复制艾滋病毒的特异性检测。
Tags
HIV-1
Marking HIV-positive Cells
Rev-dependent Lentiviral Vector
Green Fluorescent Protein (GFP)
HIV-responsive Expression Vectors
Long Terminal Repeat (LTR)
Tat-responsive LTR
Cellular Activation States
Local Chromatin Activity
HIV-independent Activity
LTR-based Reporter
HIV DNA Sequences
Rev-response Element
HIV Splicing Sites
Lentiviral Reporter Virus
Replicating HIV
Living Cell Populations
Green Fluorescence Protein (GFP) Expression
In Situ PCR
HIV Antigen Staining
Therapeutic Genes
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