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JoVE Journal Biology
Protokoll für die RNAi-Assays in Adult Moskitos (A. gambiae)
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Protokoll für die RNAi-Assays in Adult Moskitos (A. gambiae)

Article DOI: 10.3791/230
July 4th, 2007 •
1, 1
1Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University

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July 4th, 2007

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Reverse-genetische Ansätze haben sich als äußerst nützlich für die Bestimmung, welche Gene zugrunde liegenden Widerstand gegen Vektor Krankheitserreger in Mücken. Dieses Video-Protokoll stellt ein Verfahren durch die Dimopoulos Labor verwendet werden, um dsRNA in Anopheles gambiae Mücken, die den Malaria-Parasiten Hafen zu injizieren. Die Technik die Manipulation der Injektion Setup und Injizieren dsRNA in den Brustkorb dargestellt.

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