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Neuroscience

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JoVE Journal Neuroscience
Nucleofection und Primärkultur von embryonalen Maus-Hippocampus und kortikalen Neuronen
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Nucleofection und Primärkultur von embryonalen Maus-Hippocampus und kortikalen Neuronen

Article DOI: 10.3791/2373-v • 15:40 min • January 24th, 2011
January 24th, 2011 •
*1, *1, *1, 1
1Department of Anatomy, University of Wisconsin-Madison
* These authors contributed equally

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Dieses Protokoll beschreibt die erforderlichen Schritte zu zerlegen, zu transfizieren via Elektroporation und Kultur Maus Hippocampus und kortikalen Neuronen. Kurzfristige Kulturen können für die Untersuchung der Axon Auswuchs und Beratung verwendet werden, während die langfristigen Kulturen für ein Studium der Synaptogenese und dendritischen Dorn Analyse verwendet werden können.

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Neuroscience Ausgabe 47 Hippocampus Cortex Transfektion Nukleofektion Elektroporation Primärkultur
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