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Journal
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Neuroscience
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机械操纵控制神经元轴突的发展
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Mechanical Manipulation of Neurons to Control Axonal Development
Please note that all translations are automatically generated.
Click here for the English version.
机械操纵控制神经元轴突的发展
DOI:
10.3791/2509-v
•
10:02 min
•
April 10, 2011
•
Phillip Lamoureux
,
Steven Heidemann
,
Kyle E. Miller
1
Department of Zoology
,
Michigan State University, East Lansing
Chapters
00:05
Title
00:42
Fabrication of Glass Needles and Wire Needles
02:15
Calibration of the Intermediate and Working Glass Needles
03:42
Coating Needles with Attachment Factors
04:13
Equipment Set Up and Needles Alignment
06:15
Cell Attachment
07:19
Axon Extension and Data Analysis
08:13
Representative Results for Controlling Axonal Development
09:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
应用在2-1000 microdyne范围内的神经元上的力量和直接测量是实现与高精密校准的玻璃针。这种方法可以用来控制和测量轴突发展的几个方面,包括轴突起始,轴突紧张,轴突伸长速度,和力矢量。
Tags
Mechanical Manipulation
Neurons
Control
Axonal Development
Glass Micro-fibers
Forces
Hookean Bending
Calibration
Equipment Requirements
Procedures
Fabricating
Treating
Cells
Force Regimes
Cell Types
Flexibility
Methodology
Investigation
Visualization
Direct Intervention
Cellular Events
Stimulation
Regulation
Axonal Growth
Retraction
Detachment
Article
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