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从小鼠胚胎肾肾区细胞的分离和培养
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
DOI:

08:06 min

April 22, 2011

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Chapters

  • 00:05Title
  • 01:15Kidney Dissection and Capsule Removal
  • 03:23Enzymatic Digestion of Kidneys and Isolation of Nephrogenic Zone Cells
  • 06:30Phase Contrast and Fluorescent Microscopy of Purified Nephrogenic Zone Cells
  • 07:24Conclusion

Summary

Automatic Translation

在这份报告中,我们描述了一种从小鼠胚胎肾,可用于研究规范发展肾干/祖细胞的信号转导通路的祖细胞小生境的隔离和文化的方法。这些培养的​​细胞是高度开放的小分子和重组蛋白治疗,更重要的还的病毒转导,这使得候选人途径的有效操纵。

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