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从小鼠胚胎肾肾区细胞的分离和培养
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JoVE Journal Biology
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
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从小鼠胚胎肾肾区细胞的分离和培养

DOI:
10.3791/2555-v

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08:06 min

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April 22, 2011

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, , , , , ,
1Department of Molecular Medicine,Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy,Lund University Hospital

Chapters

  • 00:05Title
  • 01:15Kidney Dissection and Capsule Removal
  • 03:23Enzymatic Digestion of Kidneys and Isolation of Nephrogenic Zone Cells
  • 06:30Phase Contrast and Fluorescent Microscopy of Purified Nephrogenic Zone Cells
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

在这份报告中，我们描述了一种从小鼠胚胎肾，可用于研究规范发展肾干/祖细胞的信号转导通路的祖细胞小生境的隔离和文化的方法。这些培养的​​细胞是高度开放的小分子和重组蛋白治疗，更重要的还的病毒转导，这使得候选人途径的有效操纵。

Tags

IsolationCultureCellsNephrogenic ZoneEmbryonic Mouse KidneyEpithelial-mesenchymal InteractionOrganogenesisCongenital Kidney DiseaseEmbryonic Stem CellsRegenerative MedicineGenetic StudiesPathwaysCatalog Of Gene TranscriptionOrgan CultureCandidate ProteinsSiRNAMorpholinosSignalingStem/progenitor Cell DifferentiationRenewal

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