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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Fluorescens återhämtning efter fotoblekning (FRAP) av fluorescens märkta proteiner i Dendritutskotten hos odlade hippocampus nervceller
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Fluorescens återhämtning efter fotoblekning (FRAP) av fluorescens märkta proteiner i Dendritutskotten hos odlade hippocampus nervceller

Article DOI: 10.3791/2568
April 16th, 2011 •
1, 1, 1, 1
1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda

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April 16th, 2011

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Click here for the English version.

FRAP har använts för att kvantifiera rörlighet grön fluorescens protein (GFP)-märkta proteiner i odlade celler. Vi undersökte den mobila / orörliga fraktioner av GFP genom att analysera fluorescens återhämtning procent efter fotoblekning. I denna studie var FRAP utförs på ryggar av hippocampus neuroner.

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