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Elektrisk felt-kontrollert Regissert Migrasjon av Neural stamceller i 2D og 3D miljøer
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
DOI:

11:15 min

February 16, 2012

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Chapters

  • 00:05Title
  • 01:39Preparation of the Electrostatic Chamber
  • 02:54Neural Progenitor Cell Isolation
  • 04:09Application of the Electric Field to the Electrostatic Chamber
  • 07:18Preparation of the Organotypic Spinal Cord Slice
  • 08:40Injection of Hoechst 33342 Labeled Neural Progenitor Cells into the Organotypic Spinal Cord Slice
  • 09:38Results: Neural Progenitor Cells Show Directed Migration in the Electric Field and the Organotypic Spinal Cord Slice
  • 10:46Conclusion

Summary

Automatic Translation

Denne protokollen demonstrerer metoder som brukes til å etablere 2D og 3D miljøer i spesialdesignede electrotactic kamre, som kan spore celler<em> In vivo / ex vivo</em> Med time-lapse opptak på celle-nivå, for å undersøke galvanotaxis / electrotaxis og andre cellulære responser til likestrøm (DC) elektriske felt (EFS).

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