Journal
/
/
神经元的初步培养体系的研究疱疹病毒潜伏期和活化
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
DOI:

12:22 min

April 02, 2012

, , , , , ,

Chapters

  • 00:05Title
  • 01:52Isolation and Culture of SCG Neurons from Rat Embryos
  • 05:56Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
  • 08:35Reactivation and Assessment
  • 09:45Results: HSV-1 Reactivation
  • 11:19Conclusion

Summary

Automatic Translation

协议描述了一种高效和可重复性的模型系统研究单纯疱疹病毒1型(HSV-1)延迟和重新启动。该法采用同质交感神经神经元文化,并允许使用各种工具,包括RNA干扰和重组蛋白表达的病毒神经元相互作用的分子解剖。

Related Videos

Read Article