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En primær Neuron Kultur System for Studiet af Herpes Simplex Virus Latency og genaktivering
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1Department of Microbiology,New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine,New York University School of Medicine, 3Department of Otolaryngology,New York University School of Medicine, 4Department of Cell Biology,New York University School of Medicine, 5Department of Physiology and Neuroscience,New York University School of Medicine, 6Department of Psychiatry,New York University School of Medicine, 7Center for Neural Science,New York University School of Medicine
Chapters
- 00:05Title
- 01:52Isolation and Culture of SCG Neurons from Rat Embryos
- 05:56Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
- 08:35Reactivation and Assessment
- 09:45Results: HSV-1 Reactivation
- 11:19Conclusion
Protokollen beskriver en effektiv og reproducerbar model til undersøgelse herpes simplex-virus type 1 (HSV-1) latens og reaktivering. Assayet anvender homogene sympatiske neuron kulturer og tillader den molekylære dissektion af virus-neuron interaktioner med en række værktøjer, herunder RNA-interferens og ekspression af rekombinante proteiner.
