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迭代优化的DNA双链的SeqA-DNA复合物的结晶
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JoVE Journal Biology
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
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迭代优化的DNA双链的SeqA-DNA复合物的结晶

DOI:
10.3791/4266-v

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11:42 min

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November 01, 2012

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1Department of Biochemistry and Biomedical Sciences,McMaster University

Chapters

  • 00:05Title
  • 01:23Protein Expression and Purification
  • 03:41DNA Purification
  • 07:35Protein-DNA Complex Formation and Analysis
  • 08:35Results: Crystal Structure of SeqA delta(41-59)-A25R Bound to Hemimethylated DNA
  • 11:08Conclusion

Summary

Automatic Translation

Automatic Translation

蛋白质-DNA复合物的晶体结构，可以提供蛋白质的功能，机制，以及特定的相互作用的性质的洞察。这里，我们报告如何优化共结晶与双链DNA的长度，序列和两端<em>大肠杆菌</emSeqA，复制起始的负调控因子。

Tags

DNA DuplexesCrystallizationSeqA-DNA ComplexesEscherichia Coli SeqANegative RegulatorDNA ReplicationPremature Reinitiation EventsHemimethylated GATC ClustersOrigin Of ReplicationReplication ForksHigher Ordered StructuresHigh Affinity ComplexesMinimal Functional UnitDimerGATC SequencesMolecular LevelStructural AnalysisProtein-DNA CrystallizationPacking InteractionsProtein-protein Interactions

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