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Time-lapse imaging di fluorescenza della crescita Root Arabidopsis con manipolazione accelerato dell'ambiente root usando il RootChip
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal Bioengineering
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
DOI:

13:54 min

July 07, 2012

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Chapters

  • 00:05Title
  • 03:24Step 1: Preparation of Growth Cones for Seed Germination
  • 03:58Step 2: Seed Germination and Seedling Growth
  • 05:02Step 3: Transfer of Seedlings onto the RootChip
  • 06:51Step 4: Connecting the RootChip to the Carrier
  • 08:11Step 5: Mounting the RootChip at the Microscope
  • 09:18Step 6: Operating the RootChip using the LabView Interface
  • 11:09Results
  • 12:43Conclusion

Summary

Automatic Translation

Questo articolo fornisce un protocollo per la coltivazione di piantine di Arabidopsis in RootChip, una piattaforma di imaging microfluidico che combina controllo automatizzato delle condizioni di crescita con il monitoraggio radice al microscopio e FRET-based misura i livelli di metaboliti intracellulari.

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