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Immunology and Infection
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对小鼠诺沃克类病毒的空斑法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Plaque Assay for Murine Norovirus
Please note that all translations are automatically generated.
Click here for the English version.
对小鼠诺沃克类病毒的空斑法
DOI:
10.3791/4297-v
•
10:53 min
•
August 22, 2012
•
Mariam B. Gonzalez-Hernandez
,
Juliana Bragazzi Cunha
,
Christiane E. Wobus
1
Department of Microbiology and Immunology
,
University of Michigan, Ann Arbor
Chapters
00:05
Title
01:50
Culturing the Macrophage Cell Line RAW 264.7
03:52
Infection of RAW 264.7 Cells with MNV Inoculum
06:16
Preparation of Low Melting Point Agarose Overlay
07:34
Visualization of Plaques by Neutral Red Staining
08:49
Results: Images of Cell Monolayers Before and After Infection and Plaque Formation
09:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种方法来量化小鼠诺罗病毒(MNV),这是唯一的,有效地复制在细胞培养中的诺罗病毒的感染性颗粒。噬斑分析MNV小鼠的巨噬细胞的趋利用,可适于使用的生物或环境样品,含有MNV。
Tags
MNV
Plaque Assay
Murine Norovirus
Cell Lysis
Cytopathic Effect
Infectious Particles
Quantification
Confluent Cell Monolayer
Holes In Cell Monolayer
Plaques
Viral Infections
QRT-PCR
Fluorescent Focus Assay
MNV Antigen
Immunostained
Titrating Viruses
Focus-forming Units
Antibodies
Microscope
TCID50
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