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Chapters
- 00:05Title
- 02:43Treatment of Cells with Thrombin, RNA Isolation, Quality Assessment and Quantification of RNA
- 03:54Library Construction and Sequencing
- 08:22Data Analysis
- 12:16Results: RNA-seq Analysis Output
- 17:16Conclusion
Questo protocollo presenta una procedura completa e dettagliata di applicare RNA-seq, una potente tecnologia di nuova generazione di sequenziamento del DNA, per trascrittomi profilo in umano cellule endoteliali microvascolari polmonari con o senza trattamento trombina. Questo protocollo è generalizzabile a varie cellule o tessuti colpiti da diversi reagenti o stati di malattia.
Tags
RNA-seq AnalysisTranscriptomesThrombin-treatedControlHuman Pulmonary Microvascular Endothelial CellsGene ExpressionMRNA LevelsExternal SignalsDrug TreatmentHealthy StateDiseased StateNext-generation DNA Sequencing TechnologyRNA-sequencingCatalog TranscriptsTranscriptional StructureExpressed GenesChanging Expression LevelsComplete Transcriptome ProfilingDigital Type DatumCopy Number Of TranscriptGenomic SequenceProtocolHuman Pulmonary Microvascular Endothelial Cells With Thrombin Treatment
