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RNA-seq的凝血酶处理和控制人肺微血管内皮细胞的转录组分析
JoVE Journal
Biology
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JoVE Journal Biology
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
DOI:

18:30 min

February 13, 2013

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Chapters

  • 00:05Title
  • 02:43Treatment of Cells with Thrombin, RNA Isolation, Quality Assessment and Quantification of RNA
  • 03:54Library Construction and Sequencing
  • 08:22Data Analysis
  • 12:16Results: RNA-seq Analysis Output
  • 17:16Conclusion

Summary

Automatic Translation

该协议提供了一个完整而详细的程序,申请一个功能强大的下一代DNA测序技术,RNA-seq技术,在人肺微血管内皮细胞或无凝血酶治疗的个人资料转录。这个协议是一般化到各种细胞或组织中的影响不同的试剂或疾病状态。

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