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Chapters
- 00:05Title
- 02:43Treatment of Cells with Thrombin, RNA Isolation, Quality Assessment and Quantification of RNA
- 03:54Library Construction and Sequencing
- 08:22Data Analysis
- 12:16Results: RNA-seq Analysis Output
- 17:16Conclusion
This protocol presents a complete and detailed procedure to apply RNA-seq, a powerful next-generation DNA sequencing technology, to profile transcriptomes in human pulmonary microvascular endothelial cells with or without thrombin treatment. This protocol is generalizable to various cells or tissues affected by different reagents or disease states.
Tags
RNA-seq AnalysisTranscriptomesThrombin-treatedControlHuman Pulmonary Microvascular Endothelial CellsGene ExpressionMRNA LevelsExternal SignalsDrug TreatmentHealthy StateDiseased StateNext-generation DNA Sequencing TechnologyRNA-sequencingCatalog TranscriptsTranscriptional StructureExpressed GenesChanging Expression LevelsComplete Transcriptome ProfilingDigital Type DatumCopy Number Of TranscriptGenomic SequenceProtocolHuman Pulmonary Microvascular Endothelial Cells With Thrombin Treatment
