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Chapters
- 00:05Title
- 02:43Treatment of Cells with Thrombin, RNA Isolation, Quality Assessment and Quantification of RNA
- 03:54Library Construction and Sequencing
- 08:22Data Analysis
- 12:16Results: RNA-seq Analysis Output
- 17:16Conclusion
Bu protokol ile veya trombin tedavi olmadan insan pulmoner mikrovasküler endotel hücrelerinde profili transcriptomes RNA-seq, güçlü bir nesil DNA dizileme teknolojisi, uygulamak için tam ve ayrıntılı prosedür sunar. Bu protokol, farklı reaktifler veya hastalık durumlarında etkilenen çeşitli hücreleri veya dokulara genellenebilir olduğunu.
Tags
RNA-seq AnalysisTranscriptomesThrombin-treatedControlHuman Pulmonary Microvascular Endothelial CellsGene ExpressionMRNA LevelsExternal SignalsDrug TreatmentHealthy StateDiseased StateNext-generation DNA Sequencing TechnologyRNA-sequencingCatalog TranscriptsTranscriptional StructureExpressed GenesChanging Expression LevelsComplete Transcriptome ProfilingDigital Type DatumCopy Number Of TranscriptGenomic SequenceProtocolHuman Pulmonary Microvascular Endothelial Cells With Thrombin Treatment
