Journal
/
/
结合荧光染色体复制时序<em在原位</em>杂交
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
DOI:

17:14 min

December 10, 2012

,

Chapters

  • 00:05Title
  • 01:53BrdU Incorporation: Terminal Labeling
  • 02:35Chromosome Harvest of Monolayer Cells Cultures
  • 05:53RNase Treatment and Ethanol Dehydration
  • 06:38Fluorescent Labeling BAC DNA: Nick Translation
  • 08:19In situ Hybridization
  • 12:18Capturing Images and Quantifying BrdU Incorporation
  • 14:27Results: Chromosome Replication Timing and FISH
  • 16:45Conclusion

Summary

Automatic Translation

染色体复制定时分析的定量方法进行说明。的方法,利用结合BrdU掺入荧光<em在原位</em>,杂交(FISH),以评估哺乳动物染色体的复制定时。这种技术允许在同一细胞重新排列和未重排的染色体直接比较。

Related Videos

Read Article