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Chapters
- 00:05Title
- 01:53BrdU Incorporation: Terminal Labeling
- 02:35Chromosome Harvest of Monolayer Cells Cultures
- 05:53RNase Treatment and Ethanol Dehydration
- 06:38Fluorescent Labeling BAC DNA: Nick Translation
- 08:19In situ Hybridization
- 12:18Capturing Images and Quantifying BrdU Incorporation
- 14:27Results: Chromosome Replication Timing and FISH
- 16:45Conclusion
En kvantitativ metode til analyse af kromosomreplikation timing er beskrevet. Fremgangsmåden anvender BrdU-inkorporering i kombination med fluorescerende<em> In situ</em> Hybridisering (FISH) til at vurdere replikation timingen af mammale kromosomer. Denne teknik giver mulighed for direkte sammenligning af omlejrede og un-omlejrede kromosomer i samme celle.
